Interleukin-1 single nucleotide polymorphisms as risk factors for susceptibility of inflammatory bowel disease: an Iraqi Arab population-based study

Abstract

ABSTRACTObjectives: It is aimed to determine the association between four single-nucleotide polymorphisms (SNPs) of IL1 genes (rs1800587: IL1A−889 C/T, rs16944: IL1B−511 T/C, rs2234650: IL1R1pst1 1970 T/C and rs315952: IL1RAmspa1 11100 T/C) and the two types of IBD (UC and CD) in samples of Iraqi Arab patients.Materials and Methods: Hundred Iraqi patients (66 UC and 34 CD) were genotyped for these SNPs by polymerase chain reaction-specific sequence primer (PCR-SSP) assay.Results: A significant increased frequency of IL1A−889 C allele was observed in total IBD patients compared to controls (72.0 vs. 56.5%; Odds ratio = 1.98; Corrected p = 0.001). For IL1B−511 and IL1R1pstl 1970 SNPs, the T allele frequency was significantly increased in total patients (62.0 vs. 49.1%; Odds ratio = 1.69; Corrected p = 0.02 and 65.0 vs. 46.7%; Odds ratio = 2.12; Corrected p = 1 × 10−4, respectively). Allele and genotype frequencies of IL1RAmspa 11100 SNP showed no significant variation between total patients and controls. A similar profile of was observed in UC patients, but IL1RAmspa 11100 T allele frequency was significantly increased in patients (72.7 vs. 60.2%; Odds ratio = 1.76; Corrected p = 0.05). Among CD patients, IL1A−889, IL1R1pstl 1970 and IL1RAmspa 11100 SNP allele frequencies shared their distribution in total IBD patients. For IL1B−511 SNP, allele and genotype frequencies showed no significant difference between CD patients and controls.Conclusion: These data suggests a significant role of IL1 SNPs in etiopathogenesis of IBD in Iraqi Arab population.