Interleukin-1� micro-environment promotes viability and proliferation of malignant glioma cell U87MG

Abstract

Event Abstract Back to Event Interleukin-1β micro-environment promotes viability and proliferation of malignant glioma cell U87MG Nandakumar DN1*, Hurmath F. K1 and Palaniswamy R1 1 National Institute of Mental Health and Neuro Sciences (NIMHANS), Department of Neurochemistry, India Glioma is one of the most common and Glioblastoma (GBM) is most malignant tumours of the central nervous system. It is notorious for its highly proliferative, infiltrative and invasive behaviour. Despite substantial progress in early diagnosis and combined modality therapy including surgical therapy, radiotherapy and chemotherapy have failed to drastically change survival. Due to the invasive phenotype and diffuse penetration of glioblastoma cells into normal regions of the brain, patients diagnosed with GBM have mean survival of 10 to 12 months even with combined modality therapy. Their exceptional ability to proliferate and infiltrate normal brain makes complete radical surgical resection virtually impossible and leading in almost all cases to tumor recurrence. In human glioblastomas, pro-inflammatory cytokine IL-1β is expressed and secreted at high concentration. Objective of the current study was to study the role of IL1β on the glioma proliferation and cell viability. The human glioma cell line U87MG was cultured in 5% CO2 and 95% humidified atmosphere air at 37°C in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% foetal bovine serum (FBS). Cells were trypsinized and 100000 cells were incubated with 1 ng/mL IL-1β for 24 hours in serum free media for cell viability assay. Cell viability was measured by Trypan blue dye exclusion assay. Viable cells were plated at a density of 5000 cells per well in 96-well plates and proliferation was demonstrated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. We observed significant increase in viability of cells and proliferation of glioma cells in the micro-environment of IL-1β. Inflammation is a critical component of tumour progression. This study indicates that pro-inflammatory cytokine IL-1β, enhances cell viability and proliferation of U87MG glioma cells. Acknowledgements This work was supported by NIMHANS research grant, and research grant form Neurotaskforce, Department of Bio Technology (DBT), India. Keywords: Glioma, IL-1beta, viability, proliferation, proinflammatory cytokines Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: DN N, K HF and R P (2013). Interleukin-1β micro-environment promotes viability and proliferation of malignant glioma cell U87MG. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00263 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Nandakumar DN, National Institute of Mental Health and Neuro Sciences (NIMHANS), Department of Neurochemistry, Bangalore, Karnataka, 560029, India, drnandn@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nandakumar DN Hurmath F K Palaniswamy R Google Nandakumar DN Hurmath F K Palaniswamy R Google Scholar Nandakumar DN Hurmath F K Palaniswamy R PubMed Nandakumar DN Hurmath F K Palaniswamy R Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.