Interleukin-1β in the surrounding incision tissues is involved in postoperative hyperalgesia in rats with nicotine withdrawal

Abstract

Objective To explore the contribution of interleukin (IL)-1β in surrounding incision tissues to postoperative hyperalgesia in rats with nicotine withdrawal.Methods Ninety-six SD rats were randomly divided into Naive group (n=6),NT group (n=30),IP group (n=30) and NT+IP group (n=30).The rats in NT group,IP group and NT+IP group were divided into 5 subgroups by preoperative 2 h,postoperative 2,24,48 h and 72 h,six in each subgroup.Both paw mechanical withdrawal threshold (MWT) and paw thermal withdrawal latency (TWL) were determined at above time points for every rats.The hind paw edema was also observed.The level of IL-1β in the surrounding incisional tissues was measured by Elisa method.Results Compared with IP group,foot plantar MWT and TWL in NT+IP group were significantly reduced at postoperative 2,24,48 h and 72 h (P＜0.05),with most significant decrease at postoperative 48 h (P＜0.01).Compared with IP group for each time point,IL-1β level in NT+IP group was significantly increased (P＜0.01).Compared with preoperative 2 h (19.7± 1.9) ng/L,IL-1β level at all the time points in NT+IP group were significantly increased (P＜0.01).The IL-1β level began to rise at postoperative 2 h (62.2±3.7) ng/L,peaked at postoperative 48 h (129.5±6.3) ng/L,and then decreased at postoperative 72 h.Conclusions Nicotine withdrawal can cause postoperative incisional hyperalgesia and hind paw edema in rats.IL-1β is involved in postoperative incisional hyperalgesia in rats with nicotine abstinence. Key words: Nicotine; Dependence; Withdrawal; Hyperalgesia; Interleukin-1β