Abstract

BackgroundA growing body of evidences suggests that the ovary is a site of inflammatory reactions, and thus, ovarian cells could represent sources and targets of the interleukin-1 (IL-1) system. The purpose of this study was to examine the IL-1 system gene expressions in equine granulosa cells, and to study the IL-1β content in follicular fluid during the follicle maturation. For this purpose, granulosa cells and follicular fluids were collected from the largest follicle at the early dominance stage (diameter 24 ± 3 mm) or during the preovulatory maturation phase, at T0 h, T6 h, T12 h, T24 h and T34 h after induction of ovulation. Cells were analysed by RT-PCR and follicular fluids were studied by gel electrophoresis and immunoblotting.ResultsWe demonstrated that interleukin-1β (IL-1β), interleukin-1 receptor 2 (IL-1R2) and interleukin-1 receptor antagonist (IL-1RA) genes are expressed in equine granulosa cells. We observed that the IL-1β and IL-1RA mRNA content changed in granulosa cells during the terminal follicular maturation whereas IL-1R2 mRNA did not vary. In follicular fluid, IL-1β content fluctuated few hours after induction of ovulation.ConclusionsThe expression of IL-1β gene in granulosa cells and the follicular fluid IL-1β content seem to be regulated by gonadotropins suggesting that IL-1β could be an intermediate paracrine factor involved in ovulation.

Highlights

  • A growing body of evidences suggests that the ovary is a site of inflammatory reactions, and ovarian cells could represent sources and targets of the interleukin-1 (IL-1) system

  • This study demonstrates for the first time the expression of IL-1β, interleukin-1 receptor antagonist (IL-1RA) and interleukin-1 receptor 2 (IL-1R2) genes in equine granulosa cells

  • We showed that IL-1β and IL-1RA expression in granulosa cells vary during follicular maturation, probably under the influence of gonadotropins

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Summary

Introduction

A growing body of evidences suggests that the ovary is a site of inflammatory reactions, and ovarian cells could represent sources and targets of the interleukin-1 (IL-1) system. The purpose of this study was to examine the IL-1 system gene expressions in equine granulosa cells, and to study the IL-1β content in follicular fluid during the follicle maturation. The ovary is a site of cyclic ovulation process which is widely assumed to an inflammatory reaction [5]. Rat, and human have shown that the ovary could be a site of IL-1 system member expression [7,8,9,10]. It has (page number not for citation purposes)

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