Interleukin- 1 Beta, -2, -6, and -8 Levels in Serum and Uterine Cavity Fluid During the Normal Luteal Phase

Abstract

ObjectivePro-inflammatory cytokines such as interleukins (ILs) are implicated in various normal and pathologic inflammatory reactions, angiogenesis and tissue remodeling. Normal endometrium throughout the menstrual cycle expresses many of these molecules whose expressions are regulated by ovarian steroids. ILs expression has been associated with endometrial dysfunction such as abnormal uterine bleeding. The objective of our study was to identify changes in concentration of ILs during the luteal phase in serum and uterine cavity fluid of healthy normally menstruating women.DesignProspective basic research studyMaterials and methods18 women were enrolled in a contraceptive study and underwent uterine washing with endometrial biopsy and peripheral blood sampling during a control menstrual cycle: 5 patients were evaluated during days 5-18, 6 during days 19-22 and 7 during days 23 plus from a normal menstrual cycle. The relative levels of cytokines and chemokines were determined using multiplex Beallyate kit measuring IL-1 beta, -2, -6, and -8 levels The level of detection for each chemokine/cytokine was between 2 to 6.9 pg/ml. are reported in this abstractResultsView Large Image Figure ViewerDownload (PPT)ConclusionsThe various ILs studied presented a similar profile of changes in concentration during the luteal phase in both endometrial fluid and serum. We feel that these changes reflect the influence of progesterone secretion by the corpus luteum. The results further support the usefulness of uterine washing and serum to profile local endometrial response to various contraceptives. ObjectivePro-inflammatory cytokines such as interleukins (ILs) are implicated in various normal and pathologic inflammatory reactions, angiogenesis and tissue remodeling. Normal endometrium throughout the menstrual cycle expresses many of these molecules whose expressions are regulated by ovarian steroids. ILs expression has been associated with endometrial dysfunction such as abnormal uterine bleeding. The objective of our study was to identify changes in concentration of ILs during the luteal phase in serum and uterine cavity fluid of healthy normally menstruating women. Pro-inflammatory cytokines such as interleukins (ILs) are implicated in various normal and pathologic inflammatory reactions, angiogenesis and tissue remodeling. Normal endometrium throughout the menstrual cycle expresses many of these molecules whose expressions are regulated by ovarian steroids. ILs expression has been associated with endometrial dysfunction such as abnormal uterine bleeding. The objective of our study was to identify changes in concentration of ILs during the luteal phase in serum and uterine cavity fluid of healthy normally menstruating women. DesignProspective basic research study Prospective basic research study Materials and methods18 women were enrolled in a contraceptive study and underwent uterine washing with endometrial biopsy and peripheral blood sampling during a control menstrual cycle: 5 patients were evaluated during days 5-18, 6 during days 19-22 and 7 during days 23 plus from a normal menstrual cycle. The relative levels of cytokines and chemokines were determined using multiplex Beallyate kit measuring IL-1 beta, -2, -6, and -8 levels The level of detection for each chemokine/cytokine was between 2 to 6.9 pg/ml. are reported in this abstract 18 women were enrolled in a contraceptive study and underwent uterine washing with endometrial biopsy and peripheral blood sampling during a control menstrual cycle: 5 patients were evaluated during days 5-18, 6 during days 19-22 and 7 during days 23 plus from a normal menstrual cycle. The relative levels of cytokines and chemokines were determined using multiplex Beallyate kit measuring IL-1 beta, -2, -6, and -8 levels The level of detection for each chemokine/cytokine was between 2 to 6.9 pg/ml. are reported in this abstract Results ConclusionsThe various ILs studied presented a similar profile of changes in concentration during the luteal phase in both endometrial fluid and serum. We feel that these changes reflect the influence of progesterone secretion by the corpus luteum. The results further support the usefulness of uterine washing and serum to profile local endometrial response to various contraceptives. The various ILs studied presented a similar profile of changes in concentration during the luteal phase in both endometrial fluid and serum. We feel that these changes reflect the influence of progesterone secretion by the corpus luteum. The results further support the usefulness of uterine washing and serum to profile local endometrial response to various contraceptives.