Abstract
ObjectiveThe purpose of our study is to investigate the relationship between IL-1β -31C/T (rs1143627) and -511T/C (rs16944) polymorphisms and the preeclampsia (PE), and analyze the Linkage disequilibrium (LD) and haplotype frequency of the two polymorphism loci.MethodsPolymorphisms at -31C/T and -511T/C of IL-1β were genotyped with the method of polymerase chain reaction-restriction fragment length polymorphism (PCR- RFLP) in 232 PE and 447 control subjects. Genotype and allele frequencies between case-control groups were compared by chi-square(X2) tests. Two-point LD and haplotype frequency analyses were done with the software Haploview4.2.ResultsSignificant statistical differences were found between PE and control groups regarding genotype and allele frequencies of the two polymorphisms of IL-1β (For IL-1β -31C/T: X2 = 11.478, P = 0.003; For IL-1β-511T/C: X2 = 9.687, P = 0.008). LD analysis revealed that the IL-1β -31C/T SNP was in high LD with the IL-1β-511C/T SNP(D′ = 0.92, r2 = 0.79). Both CT and TC haplotypes showed significant differences between case and control groups. Only the plasma level of Prothrombin Time had a significantly statistical difference among TT, CT and CC groups of the preeclamptic two polymorphisms of IL-1β-31C/T and -511T/C (for IL-1β-31C/T, F = 1.644, P = 0.01; F = 1.587, P = 0.016).ConclusionOur results revealed IL-1β was associated with the PE in Chinese Han population. The CT haplotype may increase the risk of PE, while haplotype TC could be considered as a protective haplotype of PE.
Highlights
Preeclampsia(PE) is a pregnancy-specific syndrome, characterized by the new onset hypertension and proteinuria after 20th week of gestation
The aim of our research is to evaluate the association between the two gene polymorphisms of IL-1b and PE
The analysis of the Linkage disequilibrium (LD) across the IL-1b indicated that the IL-1b-31C/T SNP was in high LD with the IL-1b-511C/T SNP (D9 = 0.92, r2 = 0.79)
Summary
Preeclampsia(PE) is a pregnancy-specific syndrome, characterized by the new onset hypertension and proteinuria after 20th week of gestation. PE occurs in about 5%–7% of all pregnancies in the world, which is a leading cause of the maternal and fetal mortality and morbidity [1]. Extensive efforts to evaluate the mechanisms and molecules of PE, the underlying pathogenesis of PE has not yet been fully elucidated. Epidemiological research indicated that the PE was associated with the family and genetic control, and inheritance appeared to have a major function in the pathology of this disease [2]. PE is a multiple gene disorder, affected by genetic and environmental factors, like interaction between maternal and fetal genes, which were important determinants of maternal disease susceptibility [3]. Genetic factors cannot be ignored in the pathogenesis of PE
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