Abstract
This article is referred to research article entitled “Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method” (Nakamura et al., 2016) [1].Real-time polymerase chain reaction (PCR) detection method for unauthorized genetically modified (GM) papaya (Carica papaya L.) line PRSV-YK (PRSV-YK detection method) was developed using whole genome sequence data (DDBJ Sequenced Read Archive under accession No. PRJDB3976). Interlaboratory validation datasets for PRSV-YK detection method were provided. Data indicating homogeneity of samples prepared for interlaboratory validation were included. Specificity and sensitivity test data for PRSV-YK detection method were also provided.
Highlights
Interlaboratory validation data on real-time polymerase chain reaction detection for unauthorized genetically modified papaya line PRSV-YK
This article is referred to research article entitled “Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method” (Nakamura et al, 2016) [1]
A The number in brackets indicates the total number of samples analyzed. b ( þ) 1⁄4 number of positive reactions, ( À ) 1⁄4 number of negative reactions. c The value was calculated from comparison of the expected results with the results obtained from homogeneity test
Summary
DNA purified from fresh papaya fruit was used as sample. DNA purified from GM papaya was mixed with DNA from non-GM papaya to prepare a dilution series of GM papaya DNA. Samples were prepared at three different levels of GM papaya DNA concentrations (0%, 0.05% and 0.10% [w/w]). Aliquots of the diluted DNA were placed in individual tubes. Each tube was labeled with a randomized number. Six randomly selected tubes of each analyte concentration were tested as blind
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