Interlaboratory comparison of the Doumas bilirubin reference method

Abstract

Objectives To assess the performance of the Doumas bilirubin reference method. Design and methods Ring trails using pooled patient specimens, a calibrator and human sera enriched with unconjugated bilirubin were analyzed in five laboratories using the Doumas bilirubin reference method. Results The coefficient of variation for the linear measurement range between laboratories ranged from 1–3%. Conclusions The Doumas bilirubin reference method is robust and reproducible. Bilirubin results using this method may be used in the development of more accurate and reliable calibrators.