Interlaboratory comparison of a 10‐day sediment toxicity test method using Ampelisca Abdita, Eohaustorius Estuarius and Leptocheirus Plumulosus

Abstract

Estuarine and marine amphipods have been chosen by the U.S. Environmental Protection Agency (EPA) for use in standardized 10-d sediment toxicity tests. An interlaboratory comparison (round robin) was conducted to evaluate the precision of these methods. This comparison included three species, Ampelisca abdita, Eohaustorius estuarius, and Leptocheirus plumulosus. Each species was exposed for 10 d under static, nonrenewal conditions to four sediment treatments using standardized, species-specific test protocols by at least six independent facilities. Sediment treatments were selected for each species to include one negative control sediment and three contaminated sediments. Highly contaminated sediment from Black Rock Harbor (BRH), Connecticut, was diluted with species-specific, noncontaminated control sediment, creating test sediments that ranged in relative contamination from low to high. Laboratories showed strong, logical agreement in rank survival for all species, with control sediment consistently exhibiting the highest survival, and sediment with the greatest proportion of BRH consistently exhibiting the lowest survival. Although instances of considerable interlaboratory variability occurred, laboratories showed acceptable survival and variability in control sediments, significant agreement in ranking sediment toxicity, and agreement in the categorization of sediments as toxic or nontoxic for all three species.