Abstract
Medicines from the group of interferon inducers (IFNs) "swith on" the synthesis of type 1 interferons(IFN-I) and induce the expression of IFN-stimulated genes (ISGs) that regulate innate immunity reactions andprotect the host from infectious agents and the tumour pathology.The purpose of the study was to determine the role of the drug celagrip (CA) in the activation of innate immunity genesand the effect on the production of reactive oxygen species (ROS) in patients with follicular lymphoma (FL). to study the intensity of ROS production and the level of expression of the IFN-α2, IFN-λ1, ISG15, BCL2, P53(TP53) andUSP18 genes in response to the treatment of blood cells of patients with FL with the preparation of CA. The study involved primary cancer patients diagnosed with follicular lymphoma (FL)and healthy volunteers. A kinetic analysis of the dynamics of production of reactive oxygen species (ROS) wasperformed in whose blood cells, and the expression of the group of genes was determined by real-time PCR inresponse to CA processing. ROS production by blood cells of patients with FL and volunteers in the presence of CAsignificantly decreased (P < 0.05). The level of gene expression of ISG15, P53(TR53) and USP 18 in the group ofpatients with FL was significantly higher than that in the group of volunteers. When treating blood cells with CA,it becomes possible to divide patients with FL into groups with a positive and negative response in accordancewith the level of expression of the USP18 gene. We divided FL patients into groups with a positive and negativeresponse in accordance with the level of USP18 gene expression after treatment of blood cells with CA. The CA drug reduces the production of ROS and simultaneously stimulates the activity of the innateimmunity genes ISG15, P53(TP53) and USP18 in the blood cells of patients with FL.
Highlights
Medicines from the group of interferon inducers (IFNs) “swith on” the synthesis of type 1 interferons (IFN-I) and induce the expression of IFN-stimulated genes (ISGs) that regulate innate immunity reactions and protect the host from infectious agents and the tumour pathology
The purpose of the study was to determine the role of the drug celagrip (CA) in the activation of innate immunity genes and the effect on the production of reactive oxygen species (ROS) in patients with follicular lymphoma (FL)
The study involved primary cancer patients diagnosed with follicular lymphoma (FL) and healthy volunteers
Summary
Наровлянский А.Н.1, Полосков В.В.1, Иванова А.М.1, Кравченко С.К.2, Бабаева Ф.Э.2, Сычевская К.А.2, Мезенцева М.В.1, Суетина И.А.1, Руссу Л.И.1, Изместьева А.В.1, Оспельникова Т.П.1, Сарымсаков А.А.3, Ершов Ф.И.1. Задачи: изучить интенсивность продукции активных форм кислорода (АФК) и уровень экспрессии генов IFN-α2, IFN-λ1, ISG15, BCL2, P53(ТР53) и USP18 в ответ на обработку ЦА клеток крови больных ФЛ. В исследовании участвовали первичные онкологические пациенты с диагнозом ФЛ и здоровые добровольцы, у которых выполнен кинетический анализ динамики продукции АФК клетками крови и определена экспрессия группы генов методом полимеразной цепной реакции в реальном времени в ответ на обработку ЦА. ЦА снижает продукцию АФК и одновременно стимулирует активность генов врожденного иммунитета ISG15, P53(ТР53) и USP18 в клетках крови больных ФЛ. – формулировка идеи, цели, задач, анализ литературы и экспериментальных данных, обсуждение, написание и оформление; Кравченко С.К., Бабаева Ф.Э., Сычевская К.А. – осуществление клинической части исследований; Полосков В.В., Иванова А.М., Мезенцева М.В., Оспельникова Т.П., Руссу Л.И., Изместьева А.В., Суетина И.А.
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