Interferon Induced by Endotoxin and Newcastle Disease Virus in Rabbit Macrophage and Kidney Cell Cultures

Abstract

SummaryInterferon induction by endotoxin and NDV in rabbit macrophages showed several differences. The former was inhibited by cortisone, and also by Act D at a concentration of 5.0 μg/ml. At lower concentrations, no effect or slight accentuation of EII production occurred. In contrast, 1.0 μg/ml Act D completely inhibited VII synthesis in rabbit macrophages, while cortisone had no effect. Also, production of EII was greater at 25 than 37°, but VII was synthesized equally well at all temperatures from 25 to 37°. These in vitro findings correlate well with published findings on the effects of cortisone, temperature, and Act D on EII and VII production in rabbits.EII and VII were active to similar extents in preventing the replication of VSV in RK cells at 30, 33, and 37°. The action of EII and VII was sensitive to inhibition by Act D at 1.0 μg/ml. EII was perhaps somewhat more sensitive than VII to 0.1 μg/ml Act D, but. only a factor of 5. This difference may be significant, but generally no major differen...