Abstract
Rotating point spread functions (PSF), such as the double helix (DH) PSF, are widely used in localization-based super-resolution imaging because of their large working depth range. In this article, we propose an interferometric DH PSF (iDH PSF) using two opposed objective lenses as in the 4Pi microscope. In the proposed iDH PSF, the super-resolution in the axial PSF is transferred to the azimuthal rotation. Moreover, we design an iDH PSF whose imaging range reaches 3 μm, which is roughly 3 times as much as that which can be obtained by using other interferometric localization-based super-resolution imaging methods.
Highlights
Rotating point spread functions (PSF), such as the double helix (DH) PSF, are widely used in localizationbased super-resolution imaging because of their large working depth range
For photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM), it has been shown that engineering the PSF of the imaging system by using the astigmatic point spread function (PSF)[2], double helix PSF (DH PSF)[3,6,7], and Airy PSF8 can enable a high localization precision over a large depth range
We propose a new kind of rotating PSF: the interferometric DH PSF
Summary
Rotating point spread functions (PSF), such as the double helix (DH) PSF, are widely used in localizationbased super-resolution imaging because of their large working depth range. For PALM and STORM, it has been shown that engineering the PSF of the imaging system by using the astigmatic point spread function (PSF)[2], double helix PSF (DH PSF)[3,6,7], and Airy PSF8 can enable a high localization precision over a large depth range. Anisotropy in the localization process affects the axial precision, making it worse than the transverse precision[6] In this manuscript, we propose a new kind of rotating PSF: the interferometric DH PSF (iDH PSF). We design an iDH PSF to enable 3D imaging over a 3 μm depth range which is around 3 times of other interferometric localization based super-resolution methods[13,14,15]
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