Interferencias por contrastes yodados en la electroforesis capilar

Abstract

Introduction The administration of iodinated contrast media may interfere with the capillary electrophoresis (CE) of serum proteins. The aim of the study was to perform an in vitro study to confirm the interference caused by lomeron ® administration followed by an in vivo study in patients undergoing coronary angiographies, evaluating the kinetics of iodinated contrast elimination. Material and methods A serum pool free from monoclonal component (MC) and with an anodyne electrophoretic pattern was prepared. lomeron ® up to a concentration of 5.4 g/100 mL was added to this pool and afterwards, four decreasing dilutions were prepared (2.70 g/100 mL, 1.35 g/100 mL, 0.67 g/100 mL and 0.33 g/100 mL); all of them were processed by CE to confirm the interference and its decreasing effect as the concentration diminishes. Blood was drawn from patients undergoing coronary angiographies, at several time intervals (5–10 min, 1, 3, 5 and 8 h post-administration of contrast). Plasma samples were processed by CE, and immunofixation (IFx) of the first sample (5–10 min) was performed in order to show that the electrophoretic image did not correspond to a MC. Results In the in vitro study, CE revealed β fraction-MC peaks, which disappeared at the 0.33 g/100 mL dilution, and 8 h after lomeron ® administration in the in vivo study (one patient). On the other hand, no image suggestive of MC was detected on the reference agarose gel electrophoresis (AGE) used for the IFx. Conclusions Peaks were observed that corresponded to an interference produced by lomeron ® in the β fraction of CE, which was not found in AGE.