Abstract
Abstract Continuing our work on the problems of congenital adrenal hyperplasia (CAH) (1, 2), we have established reference values for 17-hydroxyprogesterone (17-OHP) and androstenedione (Δ4) in amniotic fluid(AF) and done 12 successful prenatal diagnoses in families at risk for CAH (3). 17-OHP was determined in the AFs of 105 women in the second trimester of pregnancy with a kit from Sorin-Biomedica (Saluggia, Italy; tritium-labeled 17-OHP, ether extraction). In another 80 pregnant women, 17-OHP was determined with a new Sorin-Biomedica kit (iodine-labeled 17-OHP, ether extraction); a DPC Coat-A-Count kit (Diagnostic Products Corp., Los Angeles, CA), with and without ether extraction; and a CIS Bio International (Gif-sur-Yvette Cedex, France) coated-tubes kit, with and without ether extraction(Table 1).
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