Abstract
Sulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method. The concentrations of these substances which can be used without serious interference are given. Sulfhydryl and disulfide reagents yield a color with the same absorption spectrum as that caused by proteins. At moderate concentrations this interference can be overcome by running appropriate blanks. Potassium ions produce a white precipitate that can be removed without change of color intensity by centrifuging the reaction mixtures after color development.
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