Interference with the humoral immune response in diverse genetic lines of chickens: I. The Effect of Carrageenan

Abstract

Two experiments were conducted in which the effect of carrageenan (CGN) on humoral immune response of chicks selected on either high (H) or low (L) antibody production to sheep red blood cells (SRBC) was determined. H and L line chicks were injected i.p. with different doses of CGN prior to immunization with SRBC or Brucella abortus (BA). Four weeks later chicks were reimmunized with the same antigens. In general, control H and L chicks had significantly higher total anti-SRBC titers than CGN-treated chicks in primary response. Also, total anti-BA titers were significantly higher in control chicks than CGN-treated chicks on days 3 and 5 following primary immunization and on days 0 and 7 of the secondary response. Overall, the 2-mercaptoethanol (2ME)-resistant anti-SRBC titers did not differ significantly among the CGN-treated groups. However, control chicks tended to have higher anti-BA 2ME-resistant titers from day 14 p.i. of primary response on. Regardless of antigen or CGN treatment, the H line chicks had significantly higher titers than L line chicks. However, the CGN treatments did not affect the antibody response to BA nor to SRBC differently between L and H line chicks. It would appear that since CGN is cytotoxic for macrophages, selection for antibody production did not result in different abilities of the macrophages of these chicken lines to respond to an antigenic challenge.