Abstract
We demonstrate a highly sensitive imaging method combined a terahertz time-domain spectroscopy and an interference effect for label-free protein detection on a polyvinylidene difluoride membrane. The method is based on terahertz time-domain spectroscopy and uses an interference effect. Biotin is linked to the membrane using poly ethylene glycol or poly ethylene glycol methyl ether to prevent it from being washed off. Binding of the biotin with streptavidin is then observed by measuring the terahertz signal change due to the variation of the membrane refractive index. We demonstrate the detection of the binding streptavidin protein in gradually decreasing concentrations, down to 27 ng mm-2, using the image recorded at 1.5 THz.
Highlights
Many researchers have been investigating various microarray technologies for their potential to enable a comprehensive analysis of the interaction of various biomaterials
We demonstrate a highly sensitive imaging method combined a terahertz time-domain spectroscopy and an interference effect for label-free protein detection on a polyvinylidene difluoride membrane
The method is based on terahertz time-domain spectroscopy and uses an interference effect
Summary
Many researchers have been investigating various microarray technologies for their potential to enable a comprehensive analysis of the interaction of various biomaterials. Easy, and parallel detection of thousands of addressable elements in a single experiment. They can be expected to become a crucial tool for high-throughput drug discovery and life science. In these methods, label substrates, which might involve fluorescence, an enzyme reaction, or a radioisotope, are used for the detection of DNA and proteins. Label substrates, which might involve fluorescence, an enzyme reaction, or a radioisotope, are used for the detection of DNA and proteins These procedures are complex and time consuming
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