Abstract
AbstractCyclodextrin glycosyltransferases (CGTases) catalyse the cyclisation of starch dextrins to cyclodextrins and concomitantly show amylolytic activity which is commonly determined by the starch‐iodine assay. In the present study it was found that cyclodextrins interfere with this assay. Adding cyclodextrins in concentrations ranging from 0.01mM to 0.6mM to starch‐iodine complex solution resulted in a significant decrease in absorbance at 600nm due to α‐ and β‐cyclodextrin competing with starch in the iodine complex formation. The largest reduction was caused by α‐cyclodextrin. Therefore, the use of the starch‐iodine assay will result in an overestimation of the amylolytic activity of CGTases – particularly when the enzyme has a high α‐cyclodextrin production activity. In contrast, determination of the amylolytic activity by measurement of released reducing sugars with CuSO4/bicinchonate was not disturbed by cyclodextrins.
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