Abstract
Iron present in biological samples prevents the extraction of DNA by hot acid hydrolysis as used in both the Schneider and Ogur-Rosen procedures [Schneider, W. C. (1945) J. Biol. Chem. 161, 293–303; Ogur, M., and Rosen, G. (1950) Arch. Biochem. 25, 262–276.] for DNA determination. Purified DNA, which is normally hot acid hydrolyzable, is rendered nonhydrolyzable by the presence of trace (0.1 m m) quantities of iron (III). Addition of a metal ion chelator, such as EDTA, to samples before hot acid treatment of DNA overcomes the inhibitory effect of iron, and, since iron is a normal cellular component, it is recommended as a general procedure for DNA determination.
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