Abstract
It has been shown that certain conditions may arise during the investigation of the oxidation of CoA esters when the presence of ethanol and alcohol dehydrogenase interfere with the detection of specific CoA ester dehydrogenases. These conditions include 1) the presence of alcohol dehydrogenase and the specific dehydrogenase under investigation in the same crude extract or protein fraction; 2) the presence of ethanol in the assay system, and 3) the requirement of the specific dehydrogenase being assayed for NAD. Obviously, all of these factors must exist for the interference to be expressed. A simple, but effective, way to exclude ethanol when synthesizing CoA esters is to use MCF in preparing the mixed anhydrides. The data presented indicate that ECF is fully replaceable by MCF.
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