Abstract
In plant antiviral breeding, R genes encoding nucleotide-binding site and leucine-rich repeat (NB-LRR) proteins serve as major genetic resources to introduce antiviral traits to crop cultivars. However, interfamily transfer—heterologous expression in a plant belonging to a taxonomically different family from the source plant of the transferred gene—of an NB-LRR gene is difficult because it often leads to inappropriate autoimmunity that results in inhibition of plant growth or lack of resistance. We previously identified a non-NB-LRR-type resistance gene, JACALIN-TYPE LECTIN REQUIRED FOR POTEXVIRUS RESISTANCE1 (JAX1), from Arabidopsis thaliana (Brassicaceae). Here, we used a transient expression assay to demonstrate that JAX1 confers broad resistance to potexviruses including pepino mosaic virus (PepMV), which causes severe damage to greenhouse tomato production worldwide. JAX1-transgenic Nicotiana benthamiana and tomato plants in the Solanaceae family exhibited broad and a high level resistance to multiple PepMV isolates. Our results suggest that non-NB-LRR-type resistance genes, especially lectin-family genes, will be useful for interfamily transfer and are promising genetic resources for producing virus-resistant crop cultivars.
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