Abstract
Humic acid (HA) films were formed on a gold electrode surface by the self-assembly method. The HA films were used as an immobilization matrix interfacing cytochrome c (cyt c) to Au electrode surface through a dip and phase inversion process. The morphologies of the films, changes of structure and properties of the electrochemistry were investigated by scanning electron microscope (SEM), UV–Vis spectroscopy (UV–Vis) and electrochemical impedance spectroscopy (EIS) and cyclic voltammetry. The results showed that the HA film acted as a good adsorption matrix for cyt c and an excellent accelerant for the redox of cyt c which gives reversible redox peaks with a formal potential of 108 mV vs SCE in pH 7.0 phosphate buffer solution. The average surface coverage of cyt c on the HA film was about 3.49 × 10 −11 mol/cm 2, indicating a monolayer of cyt c. The electron transfer rate constant was calculated to be 1.0 s −1.
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