Interfacing Cary 219 or 210 UV-VIS Spectrophotometers to the Apple-II Plus Microcomputer

Abstract

The purpose of this study was to test whether 17β-estradiol (E2) could alter neuronal activity or responsiveness to iontophoretically applied amino acid neurotransmitters in an area not reported to contain classical E2 receptors. Such a region is the cerebellum, which was selected as a model system for these studies because it has been well characterized electrophysiologically. Extracellular activity of cerebellar Purkinje neurons was recorded from urethane-anesthetized, adult, ovariectomized rats using multibarrel glass micropipets. Spontaneous firing rate and responses of single units to microiontophoretic pulses (10 s pulses every 40 s) of GABA (10–50 nA) or glutamate (GLUT, 3–40 nA) were examined before, during and after iontophoretic (0.25 mM 17β-estradiol hemisuccinate) or jugular i.v. (100, 300 or 1000 ng/kg 17β-estradiol) administration of E2. Both modes of E2 administration resulted in a significant increase in Purkinje cell excitatory responses to GLUT, independent of the direction of change in spontaneous firing rate. This effect was seen as early as one minute after iontophoretic application of E2 and 10–40 min following i.v. E2. In all cases, recovery to the control level of response was not observed by 2 h following E2 administration.17α-E2 (300ng/kg) resulted in a less pronounced, transient increase in GLUT response, while a lower dose (100 ng/kg) did not have any effect. Prior administration of the anti-estrogen tamoxifen did not prevent any of the observed E2 effects. In addition, estrogen-priming did not alter E2-induced potentiation of GLUT responses. In contrast to the effect of E2 on GLUT responsiveness, GABA-mediated inhibition of Purkinje cells was either increased, antagonized or unchanged following E2 application. In summary, this study suggests the hypothesis that circulating levels of E2 may alter neuronal sensitivity to specific neurotransmitter substances within the cerebellar circuitry.