Interconnection of Open Circuit Potential in Blood Plasma with Oxidative Stress Parameters in Patients with Acute Poisoning

Abstract

The problem of acute poisoning of chemical etiology is relevant due to the fact that their prevalence does not decrease and continues to lead in the statistics of morbidity and mortality [1]. The most prevalent are acute poisoning with drugs, narcotic substances, varied chemical organic and inorganic substances. A dangerous group is cauterizing agents, because this group is characterized by severe course of illness and frequent development of complications. It is important that the hospital lethality of this group of acute poisonings can reach up to 30%. Acute poisoning by cauterizing agents, as a rule, is accompanied by severe disturbances of the lipid peroxidation processes (LPO) and the antioxidant system (AOS), caused by damage of the digestive and respiratory tracts. The imbalance of the LPO/AOS system can also lead to the development of oxidative stress [2]. From the electrochemical point of view, the platinum electrode open circuit potential (OCP) in biological media is the integral indicator of the balance of the oxidation-reduction system of the organism. Therefore, the study of the diagnostic capabilities of this technique for assessing the condition of patients with acute poisoning by cauterizing agents seemed perspective. In addition, this study could help establish the relationship between the magnitude of the platinum electrode OCP and the oxidative stress coefficient. It was investigated 30 patients (25 male and 5 female) with acute poisoning by cauterizing agents. OCP measurements were performed in blood plasma according to the method [3]. The oxidative stress coefficient was determined as ratio of the level of malondialdehyde (MDA) in serum to the total antioxidant activity of serum (TAS). A comparative analysis of monitoring data on the magnitude of the platinum electrode OCP and the oxidative stress coefficient showed that the direction of the changes in these values was coincided (Fig. 1). On average, the changes of OCP value was about 26 mV, in some cases reaching extremely high values, for example, in the patient A the shift in the value of the OCP was 63 mV (Fig. 1a). Moreover, a sharp shift in the values OCP to positive potentials and beyond the region for apparently healthy people (-60÷-20 mV) is the evidence of the development of complications. This phenomenon make it possible to use the OCP changes during daily monitoring for diagnostics of the patient's condition and correction of its treatment. Thus, a relationship between the value of the platinum electrode OCP in blood plasma and the oxidative stress coefficient was established. OCP values in blood plasma in patients with acute poisoning by cauterizing agents can serve as an additional diagnostic criterion for assessing the severity of oxidative stress. Public health impact of chemicals: knowns and unknowns. World Health Organization: Geneva, 2016. Belova M.V., Ilyashenko K.K., Luzhnikov Y.A. // General Reanimatology. 2009; 5 (6): 40-4. Khubutiya M.Sh., Evseev A.K., Kolesnikov V.A. et al. // Russ. J. Electrochem. 2010; 46 (5): 537-41. Figure 1