Abstract

Bacterial and mammalian preparations capable of oxidative phosphorylation can be separated into a particulate fraction containing the electron transport chains and a soluble fraction containing coupling proteins necessary for restoration of phosphorylation. The addition of coupling factors of bacterial origin to submitochondrial particles was found to replace the requirement for mammalian coupling factors for restoration of phosphorylation. Coupling factors obtained from light beef heart mitochondria were found to restore phosphorylation to the electron transport particles of Mycobacterium phlei . The level of phosphorylation restored by the heterologus coupling factors was found to be lower than that restored by the native coupling factor; however, the phosphorylation restored by either coupling factor was found to be sensitive to uncoupling agents.

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