Abstract
Abstract: The objective of this work was to evaluate the phytosanitary aspect of two accessions of maize (Zea mays) seeds from the United States introduced to Brazil, regarding the presence of Wheat mosaic virus (WMoV). Two to three weeks after sowing, symptomatic leaves were tested by Elisa using specific antiserum to WMoV. The reaction was positive, and leaf samples were analyzed by real-time PCR and amplified PCR products were sequenced. The WMoV isolates had 99 to 100% nucleotide identity with isolates from Australia and the United States. Until now, there is no report of the presence of this virus in Brazil. According to the federal law on plant protection, the plants were burned to avoid the introduction of this exotic pest in the country. The obtained results show WMoV interception in Brazil.
Highlights
Interceptação do Wheat mosaic virus (WMoV) no Brasil em sementes de milho provenientes dos Estados Unidos
WMoV is transmitted by the eriophyid wheat curl mite Aceria tosichella Keifer (Seifers et al, 1997), which is the vector for Wheat
WSMV and WMoV are often found in mixed infection (Skare et al, 2006; Navia et al, 2013)
Summary
Interceptação do Wheat mosaic virus (WMoV) no Brasil em sementes de milho provenientes dos Estados Unidos. Resumo – O objetivo deste trabalho foi avaliar o aspecto fitossanitário de dois acessos de sementes de milho dos Estados Unidos introduzidas no Brasil, quanto à presença do Wheat mosaic virus (WMoV). The causal agent is a negative-sense RNA virus in the genus Emaravirus, referred to, in the literature, as High Plains virus (HPV), Maize red stripe virus (MRSV/MRStV), or, more recently, Wheat mosaic virus (WMoV) (Stewart et al, 2013).
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