Intercellular Interactions in Peripheral Venous Blood in Practically Healthy Residents of High Latitudes.

L K Dobrodeeva,A V Samodova,K O Pashinskaya,S N Balashova,Giuseppe Piccione

doi:10.1155/2021/7086108

Abstract

The paper presents the results of studying the immunological parameters of 369 people who were practically healthy at the time of the survey, 298 women and 71 men, of which 216 people are living in the European North of the Russian Federation (173 women and 43 men) and 153 are residents of the Arctic (125 women and 28 men). The study was carried out in the morning (08:00–10:00 am). The study included the determination of the aggregation of erythrocytes, platelets, neutrophilic granulocytes, lymphocytes, hemogram study, hematological analysis, enzyme immunoassay, and flow cytometry. Statistical processing of the obtained data was carried out using the Statistica 7.0 software package (StatSoft, USA). It was found that the activity of aggregation of cells of peripheral venous blood in Arctic residents is 1.5–1.7 times higher than that in people living in more favourable climatic conditions. The frequency of registration of aggregation of erythrocytes and platelets is actually 2 times higher than the aggregation of leukocytes. Aggregation of erythrocytes is associated with an increase in the concentrations of transferrin and receptors for this transport protein. The frequency of detection of platelet aggregation is accompanied by an increase in transferrin concentrations; in cases of aggregation of nonnuclear blood cells, the content of NO2 in the blood serum is increased. Aggregation of neutrophilic granulocytes and lymphocytes is associated with an increase in the content of free adhesion molecules. Aggregation of erythrocytes and platelets is in evidence when it is necessary to trigger reactions of changes in the hemodynamics of microcirculation to increase the efficiency of oxygen and trophic supply of tissues. The adhesion of leukocytes to the endothelium determines the secretion of biologically active substances that contribute to a change in microcirculation and an increase in the migration of leukocytes into tissues for the implementation of phagocytic and cytolytic functions.

Highlights

Any cells, mobile and immobile, can interact with each other
In connection with the above, we studied intercellular interactions and the frequency of registration of aggregation of peripheral venous blood cells in people living in the European North of the Russian Federation and in the Arctic
A comparative study of the frequency of registration of aggregation of erythrocytes, platelets, neutrophilic granulocytes, and lymphocytes in practically healthy individuals living in different climatic conditions was of interest

Summary

Introduction

Any cells, mobile and immobile, can interact with each other. The interaction of cells can be manifested by sticking of cells (adhesion), gluing of cells (conglutination, agglutination, and aggregation), or the formation of rosettes. The process of reaggregation is when cells of different tissues separated by treatment with various substances (for example, proteolytic enzymes or trypsin) are attached to each other forming aggregates characteristic only for a given population of cells in a particular tissue. The purpose of cell contact is to ensure the passage of substances through the membrane that are not able to pass through it on their own. This intracellular mediator is cyclic AMP (cAMP). Adenylate cyclase converted into cAMP by ATP is localized in the plasma membrane and has a regulatory subunit on the outer surface and a catalytic subunit on the inner surface of the cell membrane.

Methods

Results

Discussion

Conclusion