Abstract
AbstractUsing 1064 nm‐excited dispersive multichannel Raman spectroscopy, we were able to measure the Raman spectra of the ethidium bromide (EB)–DNA complex in aqueous solution. Excitation at 1064 nm decreased the fluorescence due to EB to a considerable extent, so that high signal‐to‐noise ratio Raman spectra of the DNA–EB complex were obtained. The effect of the intercalation of EB into calf thymus DNA was investigated for various EB/DNA (base pair) molar ratios ranging from 1/30 to 1/3. The Raman spectral changes clearly indicated a structural transition of DNA from the B‐form to an A‐like form with increasing the EB concentration. Thus, the intercalation‐induced structural change of DNA was successfully traced by Raman spectroscopy. Copyright © 2004 John Wiley & Sons, Ltd.
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