Interactive effects of nitrogen and phosphorus on the acclimation potential of foliage photosynthetic properties of cork oak, Quercus suber, to elevated atmospheric CO2 concentrations

Abstract

AbstractLeaf gas‐exchange and chemical composition were investigated in seedlings of Quercus suber L. grown for 21 months either at elevated (700 μmol mol–1) or normal (350 μmol mol–1) ambient atmospheric CO2 concentrations, [CO2], in a sandy nutrient‐poor soil with either ‘high’ N (0.3 mol N m–3 in the irrigation solution) or with ‘low’ N (0.05 mol N m–3) and with a constant suboptimal concentration of the other macro‐ and micronutrients. Although elevated [CO2] yielded the greatest total plant biomass in ‘high’ nitrogen treatment, it resulted in lower leaf nutrient concentrations in all cases, independent of the nutrient addition regime, and in greater nonstructural carbohydrate concentrations. By contrast, nitrogen treatment did not affect foliar N concentrations, but resulted in lower phosphorus concentrations, suggesting that under lower N, P use‐efficiency in foliar biomass production was lower. Phosphorus deficiency was evident in all treatments, as photosynthesis became CO2 insensitive at intercellular CO2 concentrations larger than ≈ 300 μmol mol–1, and net assimilation rates measured at an ambient [CO2] of 350 μmol mol–1 or at 700 μmol mol–1 were not significantly different. Moreover, there was a positive correlation of foliar P with maximum Rubisco (Ribulose‐1,5‐bisphosphate carboxylase/oxygenase) carboxylase activity (Vcmax), which potentially limits photosynthesis at low [CO2], and the capacities of photosynthetic electron transport (Jmax) and phosphate utilization (Pmax), which are potentially limiting at high [CO2]. None of these potential limits was correlated with foliar nitrogen concentration, indicating that photosynthetic N use‐efficiency was directly dependent on foliar P availability. Though the tendencies were towards lower capacities of potential limitations of photosynthesis in high [CO2] grown specimens, the effects were statistically insignificant, because of (i) large within‐treatment variability related to foliar P, and (ii) small decreases in P/N ratio with increasing [CO2], resulting in balanced changes in other foliar compounds potentially limiting carbon acquisition. The results of the current study indicate that under P‐deficiency, the down‐regulation of excess biochemical capacities proceeds in a similar manner in leaves grown under normal and elevated [CO2], and also that foliar P/N ratios for optimum photosynthesis are likely to increase with increasing growth CO2 concentrations. Symbols: A, net assimilation rate (μmol m–2 s–1); Amax, light‐saturated A (μmol m–2 s–1); α, initial quantum yield at saturating [CO2] and for an incident Q (mol mol–1); [CO2], atmospheric CO2 concentration (μmol mol–1); Ci, intercellular CO2 concentration (μmol mol–1); Ca, CO2 concentration in the gas‐exchange cuvette (μmol mol–1); FB, fraction of leaf N in ‘photoenergetics’; FL, fraction of leaf N in light harvesting; FR, fraction of leaf N in Rubisco; Γ*, CO2 compensation concentration in the absence of Rd (μmol mol–1); Jmax*, capacity for photosynthetic electron transport; Jmc, capacity for photosynthetic electron transport per unit cytochrome f (mol e–[mol cyt f]–1 s–1); Kc, Michaelis‐Menten constant for carboxylation (μmol mol–1); Ko, Michaelis‐Menten constant for oxygenation (mmol mol–1); MA, leaf dry mass per area (g m–2); O, intercellular oxygen concentration (mmol mol–1); [Pi], concentration of inorganic phosphate (mM); Pmax*, capacity for phosphate utilization; Q, photosynthetically active quantum flux density (μmol m–2 s–1); Rd*, day respiration (CO2 evolution from nonphotorespiratory processes continuing in the light); Rubisco, ribulose‐1,5‐bisphosphate carboxylase/oxygenase; RUBP, ribulose‐1,5‐bisphosphate; Tl, leaf temperature (°C); UTPU*, rate of triose phosphate utilization; Vcmax*, maximum Rubisco carboxylase activity; Vcr, specific activity of Rubisco (μmol CO2[g Rubisco]–1 s–1]*given in either μmol m–2 s–1 or in μmol g–1 s–1 as described in the text.