Interactive effects of dietary protein concentration, a mono-component exogenous protease and ascorbic acid on broiler performance, nutritional status and gut health

Abstract

A total of 640 male Ross 308 broiler chickens were used to explore the interactive effects of diet nutrient density, exogenous protease and ascorbic acid on performance, nutrient digestibility and various gut health metrics. A total of eight dietary treatments were arranged as a 2 × 2 × 2 factorial with the factors being adequate or reduced (~4%) crude protein and amino acids, without or with exogenous protease and without or with supplemental ascorbic acid. Over the 35-day experimental period, birds that received the adequate diet had higher (P < 0.01) bodyweight gain and lower (P < 0.001) feed conversion ratio (FCR) than birds that received the diet with reduced protein and amino acid density. Supplemental protease reduced (P < 0.001) FCR and this influence was more apparent in birds fed the diet with reduced protein concentration. Gut tensile strength tended to be increased by protease (P = 0.09) and ascorbic acid (P = 0.06) supplementation. Supplemental protease reduced (P < 0.001) jejunal goblet cell numbers and epithelial thickness and increased (P < 0.05) villus height compared with unsupplemented diets. Ascorbic acid tended (P = 0.05) to increase sialic acid concentration in ileal digesta. Protease increased (P < 0.01) the coefficients of apparent ileal digestibility for all amino acids other than methionine and this effect tended (P = 0.07 to 0.09) to be more pronounced in the low protein diet for aspartic acid, histidine and arginine. The concentration of taurine in ileal digesta was reduced (P < 0.01) by protease whereas hydroxyproline concentrations tended (P = 0.09) to increase by ascorbic acid addition. These results confirm previous reports on the effectiveness of exogenous protease in diets reduced in crude protein and digestible amino acids. Furthermore, both protease and ascorbic acid may influence gut health through promotion of tensile strength, epithelial morphology and endogenous protein flow. The interaction between exogenous protease and ascorbic acid on gut health with an emphasis on collagen structure and tight junction integrity is an area for future study.