Interactive effect of temperature and water stress on physiological and biochemical processes in soybean.

Abstract

Drought and heat stress are important abiotic stress restricting plant growth, while the two stresses often occur at the same time in nature and little is known about when these stresses occur in combination. Therefore, attempts were made to understand the impact of water stress imposed under different temperature conditions on photosynthesis, chlorophyll fluorescence, antioxidant enzymes, lipid peroxidation, chlorophyll, proline, free amino acid, epicuticular wax content and seed yield. Soybean genotype EC 538828 was grown under greenhouse conditions at day/night temperatures of 30/22, 34/24, 38/26 and 42/28°C. At each temperature, pots were divided into two sets, one set was unstressed while second was subjected to water stress at reproductive stage (beginning of seed fill). High temperature significantly declined the rate of photosynthesis, stomatal conductance, water use efficiency, Fv/Fm ratio, photochemical quenching, PhiPSII, electron transport rate, ascorbic acid, total free amino acids, chlorophyll content and seed yield. As against this with increase in temperature from 30/22 to 42/28°C intercellular CO2, transpiration rate, vapor pressure deficit, non photochemical quenching, proline content, SOD, POD, APX, GR, MDA and epicuticular wax content were increased. Water stress when imposed at different temperature further aggravated the effects of temperature, and the combination of water stress and high temperature had more detrimental effect.