Interactions of polysaccharides extracted by mild acid hydrolysis from the leaves of Sphagnum papillosum with either phenylhydrazine, o-phenylenediamine and its oxidation products or collagen

Abstract

The purpose of this research was to evaluate if pectin-like polysaccharides, collectively known as sphagnan, extracted by acid hydrolysis from the leaves of Sphagnum moss have a unique ability to react with phenylhydrazine, o-phenylenediamine or collagen. A previous assay for determination of carbonyl groups in sphagnan by reaction with phenylhydrazine was disproved due to spectrophotometric interference from furans. The actual carbonyl content of sphagnan is estimated to be much less than previously thought. NMR spectroscopy showed that small amounts of o-phenylenediamine and/or its oxidation products bind to sphagnan probably via imine formation, but evidence of quinoxaline formation was inconclusive. Sphagnan–gelatin mixtures formed complex coacervates at pH 2.0–4.8 at low ionic strength, which is typical of electrostatic polyelectrolyte interactions, rather than covalent carbonyl-amine reactions. Measurements of hydrothermal stability and collagenase-degradation of sphagnan-treated hide powder collagen suggest that sphagnan is a poor tanning agent. The results indicate the suggested preservative properties of sphagnan are not related to tanning.