Interactions of neural-like cells with 3D-printed polycaprolactone with different inner diameters for neural regeneration

Abstract

Background/purposePeripheral neural regeneration is an interesting and challenging field. The aim of this study was to investigate the interactions of neural-like PC12 cells and Poly-D-Lysine (PDL)-coated 3D-printed polycaprolactone (PCL) scaffolds with different inner diameters of half tubular array (HTA) (0, 200, 300, and 400 μm), respectively. Materials and methodsThis study used the fused deposition modeling (FDM) technique with 3D-printing to fabricate the thermoplastic polymer. Scaffold properties were measured by mechanical testing, and coating quality was observed under a scanning electron microscope (SEM). PC12 cell biocompatibility was examined by an MTT assay. Cell differentiation was evaluated by immunofluorescence staining. ResultsThe cell viability of PC12 cells on PDL-coated PCL scaffolds with a 200-μm inner diameter of HTA was shown with significant differences (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001) than other PCL groups at all experimental dates. The SEM observation showed that PDL-coated PCL scaffolds with 200-μm inner diameters of HTA promoted cell adhesion. An immunofluorescence staining of PC12 cells on the PDL-coated PCL scaffold with a 200-μm inner diameter of the HTA group showed that it stimulated PC12 cells for neurite formation much better than the other groups. ConclusionA PDL-coated PCL scaffold with a 200-μm inner diameter of HTA can promote the growth and differentiation of PC12 cells better than other groups. It indicated that PDL-coated PCL scaffolds with a 200-μm inner diameter HTA can be used for further neural regeneration application.