Interactions of cannabinoids, tetrahydrocannabinol, cannabidiol and some active metabolites with thinner and its components.

Abstract

The interaction of cannabinoids, tetrahydrocannabinol (THC), cannabidiol (CBD) and some active metabolites with thinner and its components was studied in mice and the following results were obtained. 1) Δ9-THC but not CBD (5 mg/kg, i.v.) significantly prolonged thinner-induced anesthesia by 1.8 fold. The latent period to induce the anesthesia was significantly shortened by Δ9-THC. 2) 11-Hydroxy-Δ8-THC and 11-oxo-Δ8-THC (5 mg/kg, i.v.), which are active metabolites of Δ8-THC, significantly prolonged thinner-induced anesthesia by 2.0 and 3.2 fold, respectively. 11-Oxo-Δ8-THC significantly shortened latent period to induce the anesthesia. 3) Among cannabinoids examined, only 11-hydroxy-Δ8-THC significantly prolonged toluene-induced anesthesia by 2.4 fold. Thinner contains about 60% of toluene and a small amount of ethyl acetate, ethylether and ethylalcohol. 4) Δ8-THC and its active metabolites, 11-hydroxy-Δ8-THC and 11-oxo-Δ8-THC significantly prolonged ethyl acetate-induced anesthesia by 4.4, 4.9 and 5.1 fold, respectively. Moreover, Δ8-THC, 11-hydroxy-Δ8-THC and 11-oxo-Δ8-THC significantly shortened latent period to induce anesthesia.