Abstract
The interaction of a zwitterionic surfactant with the water treatment protein extracted from Moringa oleifera has been investigated by surface tension and ultrasonic velocity measurements. The critical micelle concentration (CMC) of zwitterionic surfactant was determined to be 2.4 ± 0.3 mM by both techniques and the partial specific volume ν = 0.78 ± 0.06 cm3/g for the protein was found. There seems to be a mild interaction between the protein and the surfactant as shown by surface tension measurements. The ultrasonic velocity was found to decrease in the vicinity of the critical micelle concentration which may be due to micelle aggregates formation and the protein caused a shift of the surfactant’s CMC to a higher concentration.
Highlights
It is well-known that solution environment such as pH, salt and surfactant can significantly alter the behaviour and overall performance of biomolecules
In order to obtain better insight into their interactions with aqueous Moringa oleifera (MO) protein systems, we report here the results of an investigation of the interactions of a zwitterionic surfactant with a water treatment protein extracted from MO seeds, studied by surface tension and ultrasonic velocity techniques
A pure surfactant will have a net break at the critical micelle concentration (CMC) but the plots do not show a net break but rather it reduces and increases steadily, that is, it has a minimum which results from the impurity
Summary
It is well-known that solution environment such as pH, salt and surfactant can significantly alter the behaviour and overall performance of biomolecules. In our earlier research of the protein from Moringa oleifera (MO) seeds, we have used a number of techniques to study their effects on the properties of the protein in order to understand the nature and mechanism of this protein in water treatment [1,2,3,4,5,6,7]. Most of these studies have used anionic surfactants sodium dodecyl sulphate (SDS) some of them used cationic and nonanionic surfactants but not with zwitterionic surfactant. Their behavior is often described in relation to ionic and nonionic surfactants but the electrically neutral nature of zwitterionic surfactants clearly distinguishes them from ionic surfac-
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