Abstract
In microscopy the question arisesWhy employ electron beams instead of light beams to produce magnified images and the answer has to do with resolution. When doing microscopy to produce magnified image of objects, diffraction (bending of waves around narrow openings and obstacles) limits the resolution and hence the quality of image in terms of fine details one can see. The optical wavelengths from deep UV to IR are in range of hundreds of nanometers while electron beam of energy in keV have wavelengths in fractions of nanometers. The dependence of diffraction on the wavelength of the beam makes electron beam more suitable than beams of wavelengths in the optical region. The diffraction also depends on the size of the objects. A Scanning Electron Microscope (SEM) with electron beams in the keV range allows one to produce image (Fig. 1) of objects in the micro to nanometer range with relatively lower diffraction effects. Using a SEM to produce proper image requires a judicious choice of beam energy, intensity, width and proper preparation of the sample being studied. The electron beam in a SEM is nowadays generated using a field emission filament that uses ideas of quantum tunneling. Other methods are also available. The deflection of electron beam of certain energy E is accomplished by means of electromagnetic lenses. Typical E values for conventional SEM can range from as low as 2-5 keV to 20-40 keV.
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