Abstract
The interactions of phospholipid monolayers (dipalmitoyl phosphatidyl choline; DPPC and dimyristoyl phosphatidyl choline; DMPC) with volatile anesthetic isoflurane were investigated using quartz crystal microbalance (QCM) and quartz crystal impedance (QCI) methods. The quartz crystal oscillator was attached horizontally on the surface of DPPC and DMPC monolayer formed on the water surface. Physisorption of isoflurane hydrate at the DPPC monolayer surface was monitored in terms of frequency and resistance change of quartz crystal on addition of anesthetics isoflurane. Both frequency and resistance change showed the elastic nature of DPPC monolayer. Measurement of DMPC monolayer-isoflurane hydrate revealed the expandable nature of DMPC monolayer. Variation of frequency and impedance of DPPC and DMPC monolayer on addition of isoflurane which proved a two-step change has occurred at monolayer surface at isoflurane concentration of ≤8 mM that has been attributed to isoflurane aggregation at monolayer/water interface. Isoflurane hydrates formed in the process have capability to affect the interfacial properties of monolayer such as existence of structured water.
Highlights
Biomembranes have fluidic structures where proteins are buried in lipid bilayers
We have investigated physisorption interactions between phospholipid monolayers as the model membrane and anesthetic isoflurane using our home designed and assembled high sensitive quartz crystal oscillator (QCO) apparatus [15] [27]-[29], where the QCO was attached horizontally on those monolayers formed on the water surface
A specific part in which isoflurane is likely to combine is not found in DPPC molecule, the present research is observing the physisorption phenomenon of the interaction between the DPPC monolayer and isoflurane
Summary
Biomembranes have fluidic structures where proteins are buried in lipid bilayers. Lipids are composed of various kinds of phospholipids; a few of them are glycerol- and sphingophospholipids. Effectiveness and action of anesthetic agent on living organism are related to the above properties of membranes. Anesthesia agent molecules interact with lipid biomembrane [5]-[9] of the organism to inhibit sensory perception of whole of the body or part of the body of living organism. QCI can detect the viscosity or elasticity of adsorbed layers on the QCO independent of mass adsorption that has been applied to investigate the properties of adsorption or immobilization on liquid surfaces and has been applied in hybridization of DNA [23], phase transition of adsorbed Langmuir-Blodgett (LB) film [24], and precipitation denaturation of proteins [25] [26]
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