Interactions between natural organic matter (NOM) and the cationic dye toluidine blue at varying pHs and ionic strengths: Effects of NOM charges and Donnan gel potentials

Abstract

This study examined effects of charges of molecules of natural organic matter (NOM) on its interactions with organic cations represented by toluidine blue (TB). NOM was exemplified in this study by Suwannee River (SR) and South Platte (SP) NOM. TB/NOM interactions were probed in the range of pH 3 to 11, DOC concentrations 0–10.6 mg L−1 and ionic strengths 0.001–0.1 mol L−1. Changes of TB absorbance at indicative wavelengths of 640 and 550 nm located in the characteristic absorbance band of TB and their ratio R640/550 were used to quantify TB/NOM binding and the formation of TB dimers and possibly higher order aggregates in the Donnan volume of NOM molecules. These effects were interpreted based on the modeling of NOM charges and their Donnan gel potentials (ψD). The spectroscopic and modeling data showed that TB/NOM binding can be quantified via a complexation model that reflects the extent of electrostatic attraction between TB cations and anionic NOM molecules. The dependence of the apparent formation constant vs. ionic strength was shown to be qualitatively correlated with the ψD potentials of NOM molecules.