Interactions Between Microorganisms and a Seaweed-Derived Biostimulant on the Growth and Biochemical Composition of Amaranthus hybridus L.

Abstract

Natural biostimulants such as plant growth-promoting rhizobacteria (PGPRs) and seaweed extracts are used in agriculture to improve crop yield in an environmentally friendly approach. However, interaction effects between different biostimulants are seldom investigated. This study aimed to determine the combined effect of PGPRs and Kelpak® (KEL), a seaweed-derived extract, on the growth and biochemical composition of Amaranthus hybridus, a traditional leafy vegetable of South Africa. A pot trial was conducted with 6 treatments—control (distilled water), Bacillus licheniformis (BL) inoculum, Pseudomonas fluorescens (PF) inoculum, 1% KEL, and combination treatments of KEL + BL and KEL + PF. These were applied 3 times to A. hybridus seedlings at 2 weekly intervals prior to harvesting after 6 weeks. Growth parameters and biochemical content were quantified in the harvested material. Kelpak® significantly improved growth (leaf number and area, shoot and root length, and fresh weight) and chlorophyll and carotenoid content. Treatments with 2 microorganisms had a significant negative effect on growth and pigment content. However, the combination treatments significantly improved growth and photosynthetic pigment content to similar levels as the KEL treatment. The mineral content was significantly increased for most minerals when treated with PF and decreased when treated with KEL and KEL + BL. Treatment with 2 bacterial strains and KEL caused a significant decrease in superoxidase dismutase activity with an additive effect when applied in combination. This suggests stress reduction in A. hybridus. Combined use of PGPR and seaweed extracts promises to be an eco-friendly strategy to improve the production and mineral content of traditional leafy vegetables such as A. hybridus.