Abstract
AbstractLipid monolayers at the air‐water interface were used as a model system to study the specificity of protein‐lipid interactions of band 3, the main integral protein (the anion transport protein) of human erythrocyte membranes.Band 3‐protein was isolated by zonal electrophoresis (in acetic acid/H2O/sucrose mixtures) of erythrocyte membranes preextracted in 10% acetic acid and solubilized in 92% acetic acid. Afterwards, the protein was transferred into aqueous solutions and injected into the aqueous subphase of monolayers of different lipid classes. The changes in monolayer surface pressure following protein addition were used as a measure of the strength of protein‐lipid interactions.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Berichte der Bunsengesellschaft für physikalische Chemie
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
