Interactions between glucosylceramide and galactosylceramide I 3 sulfate and microstructures formed

Abstract

The monohexoside glycosphingolipids (GSLs), galactosylceramide (GalC), glucosylceramide (GluC), and their sulfated forms are abundant in cell membranes from a number of tissues. Carbohydrate–carbohydrate interactions between the head groups of some GSLs can occur across apposed membranes and may be involved in cell–cell interactions. In the present study, the ability of GluC to participate in trans interactions with galactosylceramide I 3 sulfate (CBS) was investigated by transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy. Gaucher's spleen GluC had polymorphic phase behavior; in its metastable state, it formed large wrinkled vesicles. It transformed to a stable state via an intermediate state in which the surface of the vesicles consisted of narrow ribbons. In the stable state, the narrow ribbons split off from the surface to form membrane fragments and flat and helical ribbons. The strength of the intermolecular hydrogen bonding interactions between the carbonyls increased in the order metastable<intermediate<stable state. Aqueous dispersions of GluC and CBS were combined to allow trans carbohydrate–carbohydrate interaction across apposed bilayers, or the lipids were premixed in a solvent before hydration to allow lateral cis interactions. Premixed dispersions of NFA-GluC and CBS remained in the metastable state even when incubated under stable state conditions. When NFA-GluC dispersions were combined with CBS dispersions, they had a small effect on each other's amide groups in the metastable state. Furthermore, conversion of NFA-GluC to the stable state was inhibited, although it reached the intermediate state, suggesting that some degree of trans interaction between these two lipids occurred.