Interactions between GA, auxin, and UNI expression controlling shoot ontogeny, leaf morphogenesis, and auxin response in Pisum sativum (Fabaceae): or how the uni-tac mutant is rescued.

Abstract

Leaf morphogenesis, including that of compound leaves, provides the basis for the great diversity of leaf form among higher plants. Leaf form is an important character by which plants adapt to their environment. The common garden pea provides a developmental model system for understanding leaf development in the legumes and a contrasting one for other groups of plants. We used genetic, tissue culture, and physiological methods, as well as DR5::GUS expression and qRT-PCR, to explore the interactions between the hormones gibberellic acid (GA) and auxin and Unifoliata ( UNI ) gene expression that control leaf morphogenesis in pea. Rate of increase in leaf complexity during shoot ontogeny (i.e., heteroblasty) and adult leaf complexity are controlled by GA through UNI . Leaves on greenhouse-grown uni-tac mutants are rescued by weekly GA or auxin applications. Auxin responsiveness is reduced in uni-tac shoot and root tips and in wild-type shoot tips treated with auxin transport inhibitors. GA and auxin increase UNI mRNA levels in uni-tac as well as that of other transcription factors. GA and auxin positively promote leaf dissection during leaf morphogenesis in pea by prolonging the time during which acropetally initiated pinna pairs are produced. GA-generated elaboration of leaf morphogenesis is in distinct contrast to that in other species, such as tomato and Cardamine . Instead, GA and auxin play common and supportive roles in pea leaf morphogenesis as they do in many other aspects of plant development