Interactions between cannabinoids and alcohol

Abstract

Event Abstract Back to Event Interactions between cannabinoids and alcohol Fernando Rodriguez-de-Fonseca1* 1 Hospital Carlos Haya, Avenida Carlos Haya 82, Laboratorio de Medicina Regenerativa, Fundación IMABIS, Spain The endogenous cannabinoid anandamide participates in the neuroadaptations associated with chronic ethanol exposure and serves as a link between exogenous cannabinoid administration and alcohol actions. While cannabinoid exposure favors alcohol intake, cannabinoid CB1 receptor antagonist reduces both alcohol intake and cue-induced relapse to alcohol selfadministration. Thus, intraperitoneal administration of the cannabinoid CB1 receptor antagonist SR141716A (0.03, 1.0 and 3.0 mg/kg) markedly inhibits ethanol selfadministration and conditioned reinstatement of ethanol-seeking behavior in both Wistar and Marchegian-Sardinian Alcohol preferring rats (mSp). ED50 analysis showed significantly higher sensitivity (p<0.05) to the effect of SR141716A in msP rats compared to heterogeneous Wistar rats. On the other side, acute peripheral administration of ethanol (4 gr/kg, i.p.) decreased anandamide in the cerebellum, the hippocampus and the ventral striatum, as well as in plasma and adipose tissue. Parallel decreases of a second acylethanolamide, palmithylethanolamide were observed in the brain. Effects were observed 45-90 min after ethanol administration. In vivo studies revealed that anandamide decreases were associated with a remarkable inhibition of the release of both anadamide and glutamate release in the ventral striatum. There were no changes in the expression and enzimatic activity of the main enzyme that degradates anandamide, the fatty acid amidohydrolase. Acute ethanol did not change neither the activity of n-acyltransferase, the enzyme that catalyzes the synthesis of the anandamide precursor, nor the expression of NAPE-PLD, the enzyme that releases anandamide from membrane phospholipid precursors. These results suggest that receptor-mediated release of acylethanolamide is inhibited by the acute administration of ethanol, and that this effect is not derived of increased fatty acid ethanolamide degradation. In conclusion, we provide clear evidence that the endogenous cannabinoid system participates in alcohol-seeking behavior in rats, and that alcohol directly modulates endocannabinoid production. Conference: 3rd Mediterranean Conference of Neuroscience , Alexandria, Egypt, 13 Dec - 16 Dec, 2009. Presentation Type: Oral Presentation Topic: Symposium 02 – Cannabinoids and brain reward processes Citation: Rodriguez-de-Fonseca F (2009). Interactions between cannabinoids and alcohol. Front. Neurosci. Conference Abstract: 3rd Mediterranean Conference of Neuroscience . doi: 10.3389/conf.neuro.01.2009.16.009 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 18 Nov 2009; Published Online: 18 Nov 2009. * Correspondence: Fernando Rodriguez-de-Fonseca, Hospital Carlos Haya, Avenida Carlos Haya 82, Laboratorio de Medicina Regenerativa, Fundación IMABIS, 13331 Marseille, Spain, fernando.rodriguez@ibima.eu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Fernando Rodriguez-de-Fonseca Google Fernando Rodriguez-de-Fonseca Google Scholar Fernando Rodriguez-de-Fonseca PubMed Fernando Rodriguez-de-Fonseca Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.