Interaction Study of Differently Coated Hydrophilic Superparamagnetic Iron Oxide Nanoparticles with Bovine Serum Albumin

Abstract

Magnetic Iron Oxide Nanoparticles have great scope of research and potential in biomedical fields. In their in vivo applications, the first macromolecules these nanoparticles would come in contact with, and interact in body are proteins. Physicochemical properties of the particles and protein’s nature both affect the end result of this interaction. The study summarizes the outcomes of the interaction between the protein, Bovine Serum Albumin and citric, ascorbic and glutamic acid coated hydrophilic superparamagnetic iron oxide nanoparticles. For comparison blank particles were also studied. Changes in the conformation and secondary structure of the protein were observed. All the particles caused structural changes in protein, which were determined by the charge, size and nature of the surface groups on nanoparticles. Citric acid coated particles brought about the greatest change in protein structure while the changes by glutamic acid coated particles were of lesser degree. Different spectroscopic techniques like UV-Visible, Fluorescence and Circular Dichroism were used to investigate the changes.