Abstract
The interaction of galactose-depleted guar galactomannan with agarose has been studied by optical rotation, and with xanthan using an Instron Materials Tester and a Rheometrics Mechanical Spectrometer. Samples were prepared by treatment of guar galactomannan with highly purified α- d-galactosidase. Modified guar galactomannans with a d-galactose content of 19–25%, in admixture with agarose, showed similar optical rotation changes on heating and cooling as did mixtures of agarose and carob galactomannan (23% d-galactose content). Modified samples with 13–16% d-galactose, in the presence of agarose, showed more pronounced optical rotation changes on heating and cooling, but samples with less than 13% d-galactose were only sparingly soluble even on autoclaving. The degree of interaction of galactose-depleted guar galactomannan samples with xanthan, as measured rheologically, increased as the d-galactose content decreased, paralleling the optical rotation changes with galactomannan/agarose mixtures. In the presence of xanthan, samples with a d-galactose content of 25% or less formed firm rubbery gels.
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