Interaction ofStreptomyces felleus with bromoxynil during growth on laboratory media

Abstract

Streptomyces felleus resistant to the herbicide bromoxynil (BX) took up 95 % of the initial amount of BX from the solid or liquid medium containing 100 μg of the herbicide per mL during a 5-d incubation. 50 % of the amount taken up was degraded and 45 % deposited in the cell (90 % in the cytoplasm, 10 % in the cell wall). A prolonged incubation time did not result in any further decrease of BX concentration. The addition of KC1 (the effect of NaCl was less pronounced) increased the affinity of BX for the cell wall and slowed down both the uptake and degradation of BX. Though P-14 was capable of growing at 5- to 10 times higher concentrations of BX in comparison with sensitiveStreptomyces strains, the herbicide caused its physiological (growth rate decrease, antibacterial antibiotic production, pigmentation, dehydrogenase activities), morphological and ultrastructural changes.