Interaction of zero valent copper nanoparticles with algal cells under simulated natural conditions: Particle dissolution kinetics, uptake and heteroaggregation

Abstract

Some metal-based engineered nanoparticles (ENPs) undergo fast dissolution and/or aggregation when they are released in the environment. The underlying processes are controlled by psychochemical/biological parameters of the environment and the properties of the particles. In this study, we investigated the interaction between algal cells and zero valent copper nanoparticles (Cu0-ENPs) to elucidate how the cells influence the dissolution and aggregation kinetics of the particles and how these kinetics influence the cellular uptake of Cu. Our finding showed that the concentration of dissolved Cu ([Cu]dissolved) in the supernatant of the culture media without algal cells was higher than the [Cu]dissolved in the media with algal cells. In the absence of the cells, dissolved organic matter (DOC) increased the dissolution of the particle due to increasing the stability of the particles against aggregation, thus increasing the available surface area. In the presence of algae, Cu0-ENPs heteroaggregated with the cells. Thus, the available surface area decreased over time and this resulted in a low dissolution rate of the particles. The DOC corona on the surface of the particles increased the heteroaggregation of the particles with the cells and decreases the uptake of the particles. Our findings showed that microorganisms influence the fate of ENPs in the environment, and they do so by modifying the dissolution and aggregation kinetics of the Cu0-ENPs.