Interaction of zearalenone and soybean isoflavone on the development of reproductive organs, reproductive hormones and estrogen receptor expression in prepubertal gilts

Abstract

The aim of the present study was to investigate the interactive effect of zearalenone (ZEA) and soybean isoflavone (ISO) on the development of reproductive organs, reproductive hormones and estrogen receptor expression in prepubertal gilts. Ninety 75-day-old female pigs (Duroc × Landrace × Yorkshire, 26.50 ± 0.60 kg) were randomly allocated to nine diet treatments during the 21 day study. The experiment employed a 3 × 3 factorial design using a non-soybean meal diet with addition of 0, 0.5 or 2.0 mg/kg ZEA and 0, 300 or 600 mg/kg ISO. The results indicated that diets supplemented with 600 mg/kg ISO could reduce the increased weight of the reproductive organs induced by ZEA at 2 mg/kg ( P < 0.05) while feed containing ISO and 0.5 mg/kg ZEA increased the weight of the reproductive organs compared with pigs fed diets with 0.5 mg/kg ZEA alone. Diets with ISO at 600 mg/kg reduced the large width of vulvas induced by diets with 2 mg/kg ZEA ( P < 0.05). Simultaneous provision of ZEA and ISO to prepubertal gilts increased the level of E2 at days 7 and 14, but decreased it at day 21 ( P < 0.05). Pigs simultaneously fed 2 mg/kg ZEA and 600 mg/kg ISO had the highest level of FSH ( P < 0.05). There was a significant interaction ( P < 0.05) between ZEA and ISO supplementation on the level of LH, and pigs offered diets with 2 mg/kg ZEA and 600 mg/kg ISO had the lowest level of LH on days 14 and 21. Animals supplemented simultaneously with ZEA and ISO showed higher ERα/ERβ mRNA expression compared to those offered diets containing 0.5 mg/kg ZEA alone or basal diets. However, this simultaneous supplementation resulted in a lower level of ERα/ERβ mRNA expression compared to offering diets with 2 mg/kg ZEA alone. It appears that ISO can counteract the estrogenic influence of a high dosage of ZEA (2 mg/kg). This affect might be attributed to competitive binding with estrogen receptors, thereby weakening the estrogenic effect of ZEA. Meanwhile, interactions between ZEA and ISO may interfere with the functioning of E2, FSH and LH in prepubertal gilts.