Interaction of tumor and surrounding tissue of mice inoculated B16 melanoma variants in terms of enzyme activity

Abstract

1. 1. The interactions of B16-F1 and B16-F10 tumors with their surrounding tissues in terms of enzyme activities such as cathepsin B, hemoglobin(Hb)-hydrolase, acid phosphatase, β-glucuronidase and plasminogen activator were investigated when said tumors proliferated locally and at secondary sites throughout the host's circulatory system. 2. 2. In the case of B16-F1 and B16-F10 tumor cells proliferating under the skin, statistical differences were not detected between the enzyme activities of the skin surrounding the tumors and control skin, nor between B16-F1 and B16-F10 tumors, except for β-glucuronidase. 3. 3. In the case of B16-F1 and B16-F10 tumor cells metastasizing to lung, statistical differences were detected between numerous enzyme activities of the lung tissues surrounding the tumors and control lung tissue, and also between B16-F1 and B16-F10 tumors. 4. 4. The activities of cathepsin B and acid phosphatase of lung tissue surrounding B16-F1 tumor were lower than those of the control lung. 5. 5. β-Glucuronidase activity of lung tissue surrounding B16-F10 tumor was higher than that of the control lung. 6. 6. The activities of cathepsin B, Hb-hydrolase and β-glucuronidase of the B16-F10 tumor were higher than those of the B16-F1 tumor. 7. 7. Results indicate that metastasized B16 melanoma tumor cells interact with surrounding lung tissues, and that cathepsin B, Hb-hydrolase and β-glucuronidase might play important roles in the metastasis of the malignant tumor.