Interaction of Sesbania mosaic virus movement protein with VPg and P10: implication to specificity of genome recognition.

Soumya Roy Chowdhury,Handanahal S Savithri

doi:10.1371/journal.pone.0015609

Soumya Roy Chowdhury, Handanahal S Savithri

Open Access

https://doi.org/10.1371/journal.pone.0015609

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Journal: PloS one	Publication Date: Jan 5, 2011
Citations: 36	License type: CC BY 4.0

Affiliation: Indian Institute of Science Bangalore

Abstract

Sesbania mosaic virus (SeMV) is a single strand positive-sense RNA plant virus that belongs to the genus Sobemovirus. The mechanism of cell-to-cell movement in sobemoviruses has not been well studied. With a view to identify the viral encoded ancillary proteins of SeMV that may assist in cell-to-cell movement of the virus, all the proteins encoded by SeMV genome were cloned into yeast Matchmaker system 3 and interaction studies were performed. Two proteins namely, viral protein genome linked (VPg) and a 10-kDa protein (P10) c v gft encoded by OFR 2a, were identified as possible interacting partners in addition to the viral coat protein (CP). Further characterization of these interactions revealed that the movement protein (MP) recognizes cognate RNA through interaction with VPg, which is covalently linked to the 5′ end of the RNA. Analysis of the deletion mutants delineated the domains of MP involved in the interaction with VPg and P10. This study implicates for the first time that VPg might play an important role in specific recognition of viral genome by MP in SeMV and shed light on the possible role of P10 in the viral movement.

Highlights

The primary infection of plants caused by the entry of viruses after mechanical damage to the cell wall and plasma membrane is mostly confined to a single cell
yeast 2 hybrid (Y2H) Study for identification of ancillary proteins that interact with Sesbania mosaic virus (SeMV)-movement protein (MP)
In order to identify viral encoded ancillary proteins that could interact with SeMV MP and may assist in cell to cell movement, all the genes encoded by SeMV genome (Figure 1A) were cloned in matchmaker Gal 4 two hybrid system 3

Summary

Introduction

The primary infection of plants caused by the entry of viruses after mechanical damage to the cell wall and plasma membrane is mostly confined to a single cell. In order to identify viral encoded ancillary proteins that could interact with SeMV MP and may assist in cell to cell movement, all the genes encoded by SeMV genome (Figure 1A) were cloned in matchmaker Gal 4 two hybrid system 3.

Results

Conclusion